Articular Cartilage Defects of the Knee by E. Carlos Rodrìguez-Merchán

Author:E. Carlos Rodrìguez-Merchán
Language: eng
Format: epub
Publisher: Springer Milan, Milano

7.3.7 Graft Preservation

It is currently a controversy issue. The graft may be used either fresh or frozen, but it has been shown that the graft kept cool in a suitable culture at 4 °C, shows increased survival of cartilage cells. This is vital as these cells will be in charge of maintaining the homeostasis of the extracellular matrix, and the survival of cartilage over time. Today it has been shown that the graft may be stored at the proper temperature to a maximum of 28 days after extraction, besides allowing performance of microbiologic studies, it allows finding the most appropriate receptor [19]. In addition, this transplant does not require immunosuppressive therapy because of the isolation of chondrocytes by the matrix.

Frozen or cryopreserved grafts can also be used but they are associated with lower percentage of cell viability, and they are being used less than before (kept at −40º) [1, 2].

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